Nucleofector® Basic Kit for Primary Mammalian Neurons
Catalog # CA10064-640
Supplier: LONZA PHARMA - BIOSCIENCE CA
CAS Number:
Specifications
- Description:Basic Nucleofector® kit for primary mammalian neurons
- Environmentally Preferable:
- Cat. no.:CA10064-640
- No. of Reactions:25
- Supplier No.:VPI-1003
Specifications
About this item
In addition to various cell type-specific kits for primary neurons, we also provide a cell group-specific basic Nucleofector® kit.
- Optimization can be performed within 1 experiment
- Detailed protocols guiding through the optimization procedure
- Optional result fine tuning with help of our Scientific Support Team
- Transfection efficiencies up to 92%
- Viabilities up to 80%
This kit is suited to determine the optimal transfection conditions for any mammalian neuron type that lacks a cell type specific optimized protocol for the Nucleofector® 2b device (or older generations I or II). In a first experiment a selection of different Nucleofector® programs is tested in combination with the basic Nucleofector® solution for neurons.
Applications: kit suited for fibroblasts from different mammalian species and various organs, already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc.
Delivery: Content: 2.25 ml Nucleofector® solution for primary mammalian neurons, 0.5 ml supplement 1, 30 µg pmaxGFP® vector (0.5 μg/μl in 10 mM Tris pH 8.0), 25 aluminum cuvette (100 µL), 25 single use pipettes.