Nucleofector® Human Stem Cell Kit 2

The Nucleofector® 2b device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector® kit 2 is one of our two kits suited for efficient transfection of primary human stem cells.

• High transfection efficiencies for primary cells
• Cells maintain functionality post transfection

Due to significant diversity between different human stem cell clones, we have developed two Nucleofector® kits (1 and 2) and an easy-to-use Nucleofector® starter kit. The Human Stem Cell Nucleofector® starter kit is used to determine which one of our two Nucleofector® kits is best suited for transfecting a specific primary human stem cell clone in Nucleofector® 2b device. It is suitable for 18 optimization reactions.

Applications:
Transfection of cell numbers in the range of 2×10⁵ to 2×10⁷ cells.
Low-throughput transfection in single cuvette format.

Delivery information: Content: 0.9 ml Human stem cell Nucleofector® solution 1, 0.9 ml human stem cell Nucleofector® solution 2, 2×0.2 ml supplement 1, 2×30 µg pmaxGFP® vector (0.5 μg/μl in 10 mM Tris pH 8.0), 20 aluminum cuvette (100 µl), 20 single use pipettes.