Nucleofector® Mouse Embryonic Fibroblast Starter Kit
CA10064-438
:
- Description:Mouse embryonic fibroblast starter Nucleofector® kit
- Environmentally Preferable:
- Cat. no.:CA10064-438
- No. of Reactions:10
The Nucleofector® 2b device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different MEF clones, we have developed two Nucleofector® kits (1 and 2) and an easy-to-use Nucleofector® Starter kit.
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
The mouse embryonic fibroblast Nucleofector® starter kit is used to determine which of the two Nucleofector® Kits is best suited for transfecting a specific primary mouse embryonic fibroblast (MEF) clone in Nucleofector® 2b Device.
Applications:
Transfection of cell numbers in the range of 2×10⁵ to 2×10⁷ cells.
Low-throughput transfection in single cuvette format.
: Content: 2.25 ml Nucleofector® solution for mouse embryonic fibroblast starter, 0.5 ml supplement 4, 30 µg pmaxGFP® vector (0.5 μg/μl in 10 mm tris ph 8.0), 25 aluminum cuvette (100 µl), 25 single use pipettes.