Nucleofector® Mouse Embryonic Fibroblast Kit 1
Catalog # CA10064-434
Supplier: LONZA PHARMA - BIOSCIENCE CA
CAS Number:
Specifications
- Description:Mouse embryonic fibroblast Nucleofector® kit 1
- Environmentally Preferable:
- Cat. no.:CA10064-434
- No. of Reactions:25
Specifications
About this item
The Nucleofector® 2b device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell.
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
The mouse embryonic fibroblast Nucleofector® kits are suitable for efficient transfection of primary mouse embryonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two Nucleofector® Kits (1 and 2) and an easy-to-use Nucleofector® Starter Kit to determine which of the two kits is optimal for a specific MEF clone.
Applications:
Transfection of cell numbers in the range of 2×10⁵ to 2×10⁷ cells.
Low-throughput transfection in single cuvette format.
Delivery: Content: 2.25 ml Nucleofector® solution for mouse embryonic fibroblast cells, 0.5 ml supplement 4, 30 µg pmaxGFP® vector (0.5 μg/μl in 10 mm tris pH 8.0), 25 aluminum cuvette (100 µl), 25 single use pipettes.