The basic parasite Nucleofector® kit 1 is one of our two Nucleofector® kits suited for transfecting parasites, e.g. Plasmodium berghei or Trypanosoma brucei, in Nucleofector® 2b device.

• Increased transfection efficiencies compared to standard methods, such as electroporation or particle bombardment

Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants. In humans, they can cause severe diseases, such as malaria (Plasmodium), sleeping sickness (Trypanosoma) or leishmaniasis (Leishmania). Nucleofection has proven to provide considerably higher transfection efficiencies (e.g., in Plasmodium berghei and Trypanosoma brucei) compared to standard methods, such as electroporation or particle bombardment. Due to significant genotypic and phenotypic diversity between species and life cycles, we have developed two basic parasite Nucleofector® kits (1 and 2) and an easy-to-use basic parasite Nucleofector® Starter Kit to determine which of the two kits is optimal for a specific parasite type.

The respective cell type-specific optimized protocols or recommendations are available under ‘Instructions’ or in our knowledge database. In these optimized protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Applications: Proven results for e.g. Plasmodium berghei and Trypanosoma brucei.