The cell line Nucleofector® kit V is one of our five Nucleofector® kits suited for transfecting cell lines in the Nucleofector® 2b system.

• High transfection efficiencies in difficult-to-transfect cell lines

This kit has been determined to be optimal for transfecting following cell types:
32D
3T3-L1
pre-ad
A20
A375
A7r5
AGS
ARPE-19
B16-F10
BA/F3 (DSMZ)
C2C12
C6
Capan-1
CHO [suspension]
CHO-S [suspension] (Life Technologies)
COS-1
HaCaT
HCT116
HEK293
HepG2
HL-60
HUV-EC-C
Jurkat
K-562 (DSMZ)
MCF7
MDA-MB-231
MDA-MB-468
NB-4 (DSMZ)
NCTC clone 929
Neuro-2a [N2a]
NG108-15
P19
PC-12
PC-3
Raji
Ramos
RAW 264.7
RBL-1
S49
Saos-2
Schneider‘s Drosophila Line 2
SH-SY5Y
SK-N-SH
SK-OV-3
SW480
T/C-28 a2
T/G
HA-VSMC
T-47D
THP-1
U-2 OS
Vero

The respective cell type-specific Optimized Protocol or recommendation is available under ‘Instructions’ or in our knowledge database. In this optimized protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

Applications: Transfection of cell numbers in the range of 2×10⁵ to 2×10⁷ cells, low-throughput transfection in single cuvette format.