Nucleofector® Cell Line Kit R
CA10064-584
:
- Description:Cell line Nucleofector® kit R
- Cat. no.:CA10064-584
- No. of Reactions:25
- Supplier No.:VCA-1001
The cell line Nucleofector® kit R is one of our five Nucleofector® kits suited for transfecting cell lines in the Nucleofector® 2b device.
- High transfection efficiencies in difficult-to-transfect cell lines
This kit has been determined to be optimal for transfecting following cell types:
B16-F0
BJ
COS-7
HeLa
HT-29
Hut-78
IMR-90
KG-1
L6
LNCaP
MDBK
NIH/3T3
NK-92
PANC-1
Sf9
WI-38
The respective cell type-specific Optimized Protocol or recommendation is available under ‘Instructions’ or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
Applications: Transfection of cell numbers in the range of 2×10⁵ to 2×10⁷ cells, low-throughput transfection in single cuvette format.
: Content:
2.25 ml Nucleofector® solution R
0.5 ml supplement 1
30 µg pmaxGFP® Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µl)
25 Single use pipettes
2.25 ml Nucleofector® solution R
0.5 ml supplement 1
30 µg pmaxGFP® Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µl)
25 Single use pipettes