The ToxiLight® 100% lysis reagent set is intended for use with the ToxiLight® non-destructive cytotoxicity assay (sold seperately) and is designed to give the user a 100% lysis or total adenylate kinase control.

• Highly sensitive - The ToxiLight® BioAssay Kit exploits the cyclic nature of the AK reaction whereby a small amount of the AK enzyme can generate high concentrations of ATP. This enables the detection of very subtle changes in cytotoxicity and reduces false negatives
• Non-destructive - ToxiLight® assay detects cellular AK present in cell culture supernatants, eliminating the need to lyse cells to perform the assay, and allowing multiple tests to be performed on the same sample
• Simple - Assay requires the simple addition of a single reagent, which can be added directly to wells in which cells are growing, or to a small sample of aspirated culture supernatant
• Fast - Readable signal is detectable 5 minutes after the addition of the ToxiLight® BioAssay reagent
• Flexible - Cell supernatants can be frozen with no loss of AK activity, allowing for long-term kinetic studies
• Safe - No hazardous chemicals are required

It contains the 100% Lysis Reagent, 10 ml and tris-acetate buffer, 50 ml (required volume correction for sample wells), and is suitable for 200 tests in a 96 well format.

Simply add the 100% lysis reagent to the control wells at a ratio of 1:2 (lysis reagent : sample volume), volume correct the remaining sample wells with Tris-Acetate Buffer and then incubate at room temperature for 10 minutes.

The ToxiLight® 100% lysis reagent should be included as a control at each time point within your cytotoxicity assay. After addition proceed with the standard ToxiLight® assay protocol.