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Specifications
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:MAP2
- Description:Human MAP2 ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.75 - 12 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:0.024 ng/ml
- Regulatory status:RUO
- Cat. no.:77210-582
Specifications
About this item
Human MAP2 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human MAP2 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 0.75 to 12 ng/ml
- Sensitivity: 0.024 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%
Human MAP2 ELISA kit (A310062) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human MAP2 in serum, plasma or other biological fluids. An antibody specific for MAP2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the MAP2 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-MAP2 antibody, which also binds the MAP2 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-MAP2 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-MAP2 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of MAP2 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of MAP2 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.