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FLAG Tag ELISA Kit
  76743-554
 :  ANTIBODIES.COM LLC
 :  
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FLAG Tag ELISA Kit
  76743-554
 :  ANTIBODIES.COM LLC
 :  A303885-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type:
    Competitive
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    FLAG Tag
  • Description:
    FLAG Tag ELISA kit
  • Size:
    96 tests
  • Sample type:
    Serum, plasma, tissue homogenates, and other biological fluids
  • Cross reactivity:
    FLAG Tag ELISA kit exhibits high specificity and excellent specificity for the detection of universal FLAG Tag. No significant cross-reactivity or interference between FLAG Tag and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    15.625 - 1000 ng/ml
  • Storage temperature:
    4 °C
  • Sample volume:
    50 μl
  • Sensitivity:
    9.375 ng/ml
  • Regulatory status:
    RUO
  • Cat. no.:
    76743-554

 

 

FLAG Tag ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of FLAG Tag in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-to-use ELISA kit
  • Detection range: 15.625 to 1000 ng/ml
  • Sensitivity: 9.375 ng/ml
  • Sample type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

FLAG Tag ELISA kit (A303885) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal FLAG Tag in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with FLAG Tag antigen. During the incubation, FLAG Tag present in the samples or standards competes with the fixed amount of immobilized FLAG Tag for binding sites on the Biotinylated Anti-FLAG Tag antibody. The more FLAG Tag present in a sample or standard, the less Biotinylated Anti-FLAG Tag antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-FLAG Tag antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of FLAG Tag present in each sample or standard. The concentration of FLAG Tag can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.