Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:TPSB2
- Description:Rat TPSB2 ELISA kit
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rat TPSB2 ELISA Kit exhibits high specificity and excellent specificity for the detection of rat TPSB2. No significant cross-reactivity or interference between TPSB2 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0.094 ng/ml
- Regulatory status:RUO
- Cat. no.:76733-340
Specifications
About this item
Rat TPSB2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat TPSB2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Rat TPSB2 ELISA kit (A303760) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat TPSB2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for TPSB2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TPSB2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TPSB2 Antibody, which binds the captured TPSB2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TPSB2 captured in each well. The concentration of TPSB2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.