phi29-XT RCA Kit
About this item
The phi29-XT RCA DNA amplification kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification approach to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence.
- High sensitivity: amplify from as little as 1 fg of input plasmid DNA
- Powered by phi29-XT DNA Polymerase, an engineered polymerase
- Simple workflow produces high yield in a short reaction time
- Includes exonuclease-resistant random primers and dNTPs
- Flexible protocol allows for different types of input material
RCA enables several downstream applications, including DNA sequencing, cell-free protein expression, viral DNA enrichment, plasmid amplification, and padlock probe-based assays. The kit features phi29-XT DNA polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating higher yield in a shorter reaction time than wild-type phi29 DNA polymerase.
Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any circular DNA sequence and dNTPs. Input material can be a purified plasmid, liquid media culture, a cultured colony from an agar plate, or a glycerol stock.
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
The phi29-XT RCA DNA amplification kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification approach to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence.
- High sensitivity: amplify from as little as 1 fg of input plasmid DNA
- Powered by phi29-XT DNA Polymerase, an engineered polymerase
- Simple workflow produces high yield in a short reaction time
- Includes exonuclease-resistant random primers and dNTPs
- Flexible protocol allows for different types of input material
RCA enables several downstream applications, including DNA sequencing, cell-free protein expression, viral DNA enrichment, plasmid amplification, and padlock probe-based assays. The kit features phi29-XT DNA polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating higher yield in a shorter reaction time than wild-type phi29 DNA polymerase.
Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any circular DNA sequence and dNTPs. Input material can be a purified plasmid, liquid media culture, a cultured colony from an agar plate, or a glycerol stock.
Product Family Options
Product Information
- No. of reactionsAvailabilityPrice
- CA76717-052phi29-XT RCA DNA Amplification kitSupplier Number: E1603LItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:New ProductNew Product
- CA76717-046phi29-XT RCA DNA Amplification kitSupplier Number: E1603SItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:New ProductNew Product
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Catalog #: CA21905-026
Supplier: Kimberly-Clark