The Ph.D.−12 Phage display peptide library kit v2 is based on a combinatorial library of random dodecapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

• Ready to use complex phage library (~1E09 clones)
• Monoclonal antibody and protein G magnetic beads included for new control panning experiment
• Panning against mouse mAb yields more reliable epitope results than former target protein
• Does not require helper phage for amplification
• Inherent link between phenotype and genotype allows screening of billions of clones in a single tube

The Ph.D.−12 phage display peptide library Kit v2 contains the Ph.D.−12 phage display peptide library, a DYKDDDDK mouse monoclonal antibody and protein G magnetic beads for a panning control experiment, and enough −96gIII sequencing primer for >50 sequencing reactions.