Quality control test, Indirect ELISA – to determine titer of the antibody , SDS PAGE – to determine purity of the antibody, and BCA - to determine quantity of the antibody.

Zinc-alpha-2-glycoprotein (ZAG) is found in body fluids such as serum, sweat, and seminal and breast cyst fluids. It is identical in amino acid sequence to tumor-derived lipid mobilizing factor (LMF), a protein associated with the dramatic loss of adipose body stores in cancer cachexia, and has been shown to stimulate lipolysis by adipocytes in vivo and in vitro. A role for ZAG has been proposed in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and also it regulates melanin production by normal and malignant melanocytes. It has also recently been classified as a novel adipokine in that it is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia. Controlling ZAG/LMF's activity could be life-saving in the management of certain cancers and other cachexia-inducing conditions, and its possible normal role in body fat store homeostasis is deserving of understanding in its own right. ZAG exhibits a class I major histocompatibility complex (MHC) fold but is a soluble protein rather than being anchored to plasma membranes and does not associate with alpha-2-microglobulin in humans.

Storage temperature: The lyophilized antibody remains stable and fully active until the expiry date when stored at –20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time; it does not show decline in activity after one week at 4 °C.