96 wells (1 kit) - ELISA is a competitive enzyme immunoassay for the quantitative measurement of PINP.

The IDS Rat/Mouse PINP EIA kit is a competitive enzymeimmunoassay (EIA) for the quantitative determination of N-terminal propeptide of type I procollagen (PINP) in rat or mouse serum or plasma samples. An important step in the bone formation process is synthesis of type I collagen, which is the major organic component in bone matrix. During collagen synthesis, propeptides are released from both the amino- and carboxyterminal parts of the procollagen molecule (1). These propeptides are secreted into the blood circulation, and commercially available immunoassays for their measurement from human serum have been developed. Assay for human aminoterminal propeptide of type I procollagen (PINP) is probably the most specific and sensitive marker of bone formation (2). PINP is a particularly useful marker for monitoring the efficacy of osteoporosis therapy with anabolic agents (3,4), but it is also one of the best bone turnover markers for monitoring the efficacy of antiresorptive therapy (4,5).

The IDS Rat/Mouse PINP EIA kit is a competitive enzymeimmunoassay utilising a polyclonal rabbit anti-PINP antibody coated onto the inner surface of polystyrene microtitre wells. Calibrators, controls and samples are added to the wells of the microtitre plate followed by PINP labelled with biotin and the plate incubated for 1 hour at room temperature before aspiration and washing. Enzyme (horseradish peroxidase) labelled avidin is added and binds selectively to complexed biotin and, following a further wash step, color is developed using a chromogenic substrate (TMB). The absorbance of the stopped reaction mixtures are read in a microplate plate reader, colour intensity developed being inversely proportional to the concentration of PINP.