Versatile hydrophilic stationary phase for size exclusion of peptides and proteins up to 1,250 kDa.

• Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
• Sharper peaks, higher resolution, and better protein recovery
• Faster separations than large-particle SEC columns
• Compatibility with most aqueous buffers
• Excellent stability in high-salt and low-salt conditions

Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle. Agilent Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers .