You searched for: Protein Linking and Labeling Systems

Supplier: G-Biosciences

G-Biosciences' femtoCHROMO™- AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

Supplier: Thermo Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Supplier: Thermo Scientific

The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.

Supplier: Thermo Scientific

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.

Supplier: Thermo Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

Supplier: G-Biosciences

G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

Supplier: G-Biosciences

G-Biosciences' Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

Supplier: New England Biolabs (NEB)

CLIP-Surface™ 488 is a photostable green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro

Supplier: G-Biosciences

G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups

Supplier: G-Biosciences

G-Biosciences' SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification

Supplier: New England Biolabs (NEB)

SNAP-Surface™ 549 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

Supplier: New England Biolabs (NEB)

SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

Supplier: Thermo Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Thermo Scientific

Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

Supplier: Thermo Scientific

The DyLight™ Antibody Labeling Kits and were specifically developed for fast, efficient labeling of antibodies

Supplier: Thermo Scientific

Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.

Supplier: Thermo Scientific

The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) label reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).

Supplier: Thermo Scientific

Pierce™ FITC Antibody Labeling Kit is a multi-component kit containing a high performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

Supplier: New England Biolabs (NEB)

SNAP-Surface® 594 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living or fixed cells

Supplier: New England Biolabs (NEB)

CLIP-Surface™ 647 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells, or in vitro

Supplier: G-Biosciences

G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli

Supplier: New England Biolabs (NEB)

SNAP-Cell® 430 is a blue fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

Supplier: New England Biolabs (NEB)

SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates

Supplier: New England Biolabs (NEB)

SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells

Supplier: New England Biolabs (NEB)

SNAP-Cell 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

Supplier: New England Biolabs (NEB)

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.