About this item
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.
- Label 5 to 20 µg, 20 to 50 µg, or 50 to 100 µg Ab
- Choice of 32 CF® Dye colors
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.
These kits feature a choice of 32 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.
Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye.
Biotium now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG.
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