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Specifications
- Origin:US
- Serum format:Frozen
- Description:HyClone dialyzed FBS, US Origin, 500 ml, Irradiated 25 - 40 kGy
- Size:500 ml
- Hemoglobin:≤10 mg/dl
- Endotoxin:≤10 EU/ml
- Storage temperature:Sera should be stored at −10 °C or lower. Once thawed, sera should be stored at 2...8 °C.
- Cat. no.:CA76324-878
Specifications
About this item
HyClone Dialyzed Fetal Bovine Serum is used in applications requiring serum depleted from low-molecular weight components of less than Mr 10000.
- Sourced in the USA with complete traceability back to original source
- Low in antibodies and high in growth factors
- Produced using true pool technology for uniformity and consistency from bottle to bottle
- Filtered through three sequential 100 nm (0.1 µm) pore size-rated filters
- Virus panel testing according to 9 CFR 113.53
The serum is subjected to dialysis through a diafiltration process that reduces concentrations of small molecules such as nucleotides and amino acids that are necessary for alternative biochemical survival pathways. The process reduces hypoxanthine and thymidine concentrations below detectable levels. While producing the same results, a major difference between diafiltration and traditional dialysis is that the driving force for diafiltration is hydrostatic pressure, rather than the concentration gradient for traditional dialysis. Monitoring glucose concentrations carefully controls the diafiltration process. The glucose concentration, which is representative of the extent of dialysis, is reported for each lot of serum.
Dialyzed FBS is assayed for gamma globulin, alkaline phosphatase, lactate dehydrogenase, glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, pH, total protein, albumin, blood urea nitrogen, creatinine, total bilirubin, sodium, potassium, calcium, chloride, inorganic phosphorus, osmolality, iron, total iron binding capacity (TIBC), percent iron saturation, glucose, and IgG. Included assays are subject to change without notice.