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Specifications
- Description:Fluoro-Jade C, dry powder
- Size:30 mg
- Storage conditions:The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJC is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJC dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.
The TR-160-FJC material is shipped ambient and stable at room temperature during transport.
The dry powder is stable for 12 months at room temperature if stored as recommended. The 0.1% stock solution can be stored at 2 - 8 °C for up to 3 months if handled aseptically. The 0.0001-0.0004% working solution should be used within 4 hours of preparation. - Storage temperature:Ambient temperature for powder.
- Cat. no.:76459-464
Specifications
About this item
Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
- Stains all degenerating neurons regardless of specific insult or mechanism of cell death
- Exhibits the greatest signal to background ratio, as well as the highest resolution
- Maximal contrast and affinity for degenerating neurons
- Identifies degenerating nerve cell bodies, distal dendrites, axons and terminals
The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Following our detailed protocol, Fluoro-Jade C labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade C dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
FJC visualization is accomplished using blue light or a 488 nm Laser. Excitation Peak: 495 nm; Emission Peak: 521 nm; Filter system for visualizing: Fluorescein/FITC.
Specificity: Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain.
Cross reactivity: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.