- Model:SP-400-BIO
- Absorbance range:‒0.3 - +2.5 A
- Detector:Diode array
- Light source:Xenon
- Description:UV/Visible diode array scanning spectrophotometer
- Wavelength reproducibility:±2 nm
- Wavelength noise:±0.002 A at 0.04 A, ±0.02 A at 2.0 A and 546 nm
- Photometric noise:±0.002 A at 0.04 A
- Electrical:100 - 240 VAC, 50/60 Hz
- W×D×H:21.2×42.2×12 cm (8×16×4.75")
- Path length:10 - 100 mm
- Wavelength range:198 - 800 nm
- Wavelength accuracy:±2 nm
- Output:USB
- Concentration units:±2500
- Display:Touch screen LCD
- Drift:±0.005 A/h at 0.04 A and 546 nm after 60 min. (photometric)
- Noise:±0.002 A at 0.04 A (photometric)
- Photometric accuracy:±0.01 A at 1.0 A and 546 nm
- Photometric stability:±0.005 A/h at 0.04 A and 546 nm after 60 min.
- Stray light:<1% according to ANSI/ASTM E387-72
- Cat. no.:76169-036
- Bandwidth:3 nm
Utilizes diode array technology to scan the entire wavelength range in less than 3 seconds.
Pre-programmed for DNA, RNA and protein analysis
Compatible with ultra-micro, semi-micro, micro and macro cuvettes
Scanning diode array technology, color touchscreen navigation
Small footprint and lightweight (< 3kg) fast scan speed
English, French, German, Italian and Spanish language options multiple USB ports for data storage and printer connectivity two-year warranty including xenon lamp
Dedicated to life science applications - preprogrammed for DNA, RNA and protein analysis
This simple, low cost, easy to use UV/visible spectrophotometer is dedicated for life science applications. The fast, responsive color touchscreen and pre-programmed methods for the measurement of nucleic acid concentration and purity, protein assays and cell density, makes this spectrophotometer the ideal addition to any laboratory. The spectrophotometer utilizes diode array technology to scan the entire wavelength range (198 to 800nm) in less than 3 seconds and the narrow spectral bandwidth of 3nm, makes it ideal to identify peaks for determining nucleic acid purity. The 1024 element diode array detector coupled with a flash xenon lamp results in a long life, robust spectrophotometer.
The nucleic acids measurement mode can be used to quantify the concentration and purity of dsDNA, ssDNA, RNA and oligonucleotides; using wavelengths recorded at 260, 280 and 230 nm, with an optional correction at 320 nm. The concentration is calculated along with the corresponding ratios. At the touch of a button it is easy to check the purity of the nucleic acids. This is done by identifying distorted peaks in the purity scan between 200 and 350 nm. This is especially useful for RNA samples where impurities maybe present at 230 nm, but cannot be detected using the 260/280 nm ratio measurement.
The proteins measurement mode can be used to calculate protein concentration by creating standard curves from protein assay kits. With pre-programmed methods for measuring Bradford, Lowry, Biuret and Bicinchoninic Acid (BCA) assays; up to six standards can be measured with three replicates of each standard to minimize any dilution errors. The Proteins measurement mode is also pre-programmed with the direct UV and Warburg-Christian methods to determine protein purity.
The spectrophotometer has a pre-programmed method for measuring optical density of bacterial cultures such as E.coli and yeast cells. This is ideal to measure cell growth before cell harvesting. As well as these pre-programmed methods this versatile spectrophotometer has measurement modes for simple photometrics, concentration, quantitation, spectrum scanning and kinetics. Enabling measurements to be performed at any selected wavelength between 198 to 800 nm.
Use the preprogrammed nucleic acid and protein measurement modes to calculate concentration levels. The nucleic acids measurement mode quantifies the concentration and purity of dsDNA, sDNA, RNA, and oligonucleotides. A simple touch of a button can check the purity of the nucleic acids. Calculate the protein concentration using the protein measurement mode. Preprogrammed methods for measuring Bradford, Lowry, Biuret, and Bicinchoninic Acid (BCA) assays minimize errors.
Note: The Jenway model number 720601 has now been rebranded Cole-Parmer under model number SP-400-BIO. All the same great features and specifications, just with a new and improved look.
Three years warranty.