About this item
An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats. Immobilized Metal Affinity Chromatography (IMAC) purifications employing Ni-NTA (nickel-nitrilotriacetic acid) magnetic beads can be performed under native or denaturing conditions which permit efficient binding and purification of insoluble proteins, proteins that aggregate in inclusion bodies, or proteins that possess a tertiary structure that sequester the polyhistidine affinity tag.
- NTA coordination exhibits low Nickel ion leaching
- Tolerates a wide range of conditions, including the presence of protein denaturants and detergents
- Low non-specific binding permits immobilized fusion proteins to be used in subsequent experiments
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Supplier: BDH
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: Corning
Supplier: VWR International