dUTP Solution
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Product Details & Documents
This contains 0.25 ml of 100 mM ultrapure dUTP.
- ≥99% purity as determined by HPLC analysis
- Combine with Antarctic Thermolabile UDG to prevent carryover contamination in PCR and qPCR
dUTP can either be fully substituted for dTTP or added in addition to conventional dNTPs. dUTP can be used with Family A polymerases (such as Taq) or modified Family B polymerases, such as Q5U Hot Start High-Fidelity DNA Polymerase. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases. For a simplified protocol, dUTP can be added to most polymerase reactions at the same concentration as other nucleotides (a 1:1:1:1:1 dA/dC/dG/dT/dU ratio). For applications that require other molar ratios (up to and including full substitution of dU for dT), custom nucleotide mixes must be made. We recommend starting with a concentration of 0.2 mM of each dNTP for both Taq and Q5U DNA polymerases.
Specifications
Specifications
- Description:dUTP Solution 100 mM
- Size:25 µmol
Specifications
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Catalog #: CA21905-026
Supplier: Kimberly-Clark