The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming.

• Part of the Ultra II RNA directional or non-directional workflows
• Compatible with poly(A) mRNA isolation or ribosomal RNA depletion

The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows.

This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows enable high yield preparation of high quality libraries from 5 ng to 1 g total RNA.