About this item
LunaScript RT SuperMix Kit features an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow.
- This kit is an optimized master mix for first strand cDNA synthesis in a two-step RT-qPCR workflow
- Less than 15 minute first-strand cDNA synthesis protocol
- Novel, thermostable Luna Reverse Transcriptase improves performance
- Combine with Luna qPCR master mixes for robust RT-qPCR results
- Enables consistent linearity, sensitivity, and capacity for reliable RNA quantification
It includes the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix Kit contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
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Specifications
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