The Monarch® HMW DNA extraction kit for tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian.

• Isolate DNA into the Mb size range from soft organ tissues and bacteria
• Fast, user-friendly workflows (90 minutes for tissues and bacteria)
• Obtain excellent yields of highly-pure and intact DNA; ideal for long read sequencing
• Includes RNase A and Proteinase K, as well as microtube pestles for convenient sample homogenization

The optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to ≥500 kb for the standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For tissue and bacteria, the processing time is 90 min.  Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing.