Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Size:
  100 µg
• Tag sequence:
  His Tag
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1.0 EU per μg by the LAL method.
• Biological activity:
  Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (V483A), His Tag (Cat. No. SPD-C52H5) with an affinity constant of 4.13 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (V483A), His Tag (SPD-C52H5) with an affinity constant of 6.15 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD (V483A), His Tag (Cat. No. SPD-C52H5) with an affinity constant of 4.57 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Protein synonyms:
  Spike|S glycoprotein RBD|Spike glycoprotein Receptor-binding domain|Spike protein RBD|S protein RBD|COVID-19
• Protein/peptide name:
  S protein RBD
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  27.0 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping temperature:
  RT
• Cat. no.:
  103871-318