About this item
NEBNext® Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.
- High efficiency conversion
- Minimal DNA damage
- Minimized GC bias
While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. The NEBNext® Enzymatic Methyl-seq (EM-seq™) conversion module provides an enzyme-based alternative to bisulfite conversion.
For use in Illumina® sequencing workflows, we recommend can use of the NEBNext® Enzymatic Methyl-seq (EM-seq™) kit (76337-184, 76337-038) instead of this module. The full kit includes the EM-seq™ Adaptor, which has been optimized for high performance in the EM-seq™ workflow, as well as the high-efficiency Ultra II library prep reagents, and these components are not included in the conversion module.
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