NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.

• Optimized for the EM-seq workflow
• Compatible with bisulfite sequencing workflows
• Addresses the 'barcode hopping' issue seen with some Illumina sequencing instruments
• Increases sample identification specificity with unique dual index primer pairs

The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials. Although optimized for the EM-seq™ workflow, the NEBNext EM-seq™ Adaptor is also compatible with bisulfite sequencing workflows.