igScript™ Probe-Based One Step RT-qPCR Kit provides improved PCR efficiency, wider dynamic range, superior sensitivity and specificity.

• Enhanced efficiency, specificity, and sensitivity
• Compatible with all real-time PCR instruments
• Superior gene expression results under various cycling conditions
• Robust and active for cDNA synthesis at temperatures up to 55 °C

IgScript™ Probe-Based One Step RT-qPCR 2× master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl₂, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based One Step RT-qPCR 2× master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in RT-qPCR. This 2× master mix requires minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.