For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. 

• Enables complete filtering of index-hopped reads
• Highly efficient adaptor ligation
• Minimized adaptor-dimer formation
• Includes index primers for library multiplexing
• Combine with sets 1, 2, and 4 for 384 barcodes

The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification. This third set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.

Additional NEBNext Multiplex Oligos products are available in sets of 12 and 96 single index primers as well as additional dual index primers for increased sample identification specificity.