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Specifications
- Description:Carbonyl assay kit
- Cat. no.:76423-318
- No. of Tests:100 tests
Specifications
About this item
Quantification of carbonyl groups or protein carbonyls in biological samples.
- Fast and sensitive: use of 100 µl sample, linear detection range from 12 to 250 µm carbonyl in 96-well plate assay
- The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes
- Detection limit: 12 µm
- Method: OD375nm
- Samples: chemicals with carbonyl groups or protein carbonyls in biological samples
Carbonyl groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems' carbonyl assay kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.