You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells
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Pierce™ Fluor Phosphine Reagents, DyLight™, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
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femtoCHROMO™- AP Kit for Chromogenic Detection, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' femtoCHROMO™- AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
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Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific
Supplier: Thermo Scientific
The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.
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Pierce™ Controlled Protein-Protein Cross-Linking Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.
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Peptide Coupling Kits, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' HOOK™ Peptide Coupling (Amine Reactive) Kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins
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Pierce™ PEGylated SMCC Heterobifunctional Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.
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Pierce™ Dye Removal Columns, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.
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Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific
Supplier: Thermo Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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Pierce™ Magnetic RNA-Protein Pull-Down Kit, Thermo Scientific
Supplier: Thermo Scientific
The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.
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Pierce™ Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Thermo Scientific
Supplier: Thermo Scientific
Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide
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Pierce™ EZ-Link™ Amine Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Thermo Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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CLIP-CELL® TMR-Star, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Cell™ TMR-Star is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro
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SNAP-BIOTIN®, New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Biotin®i s a cell-permeable substrate (BG-Biotin) based on biotin with an amidocaproyl linker
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Pierce™ Reduce-Imm™ Reducing Column, Thermo Scientific
Supplier: Thermo Scientific
Eliminate soluble components and reduce disulfide bonds without sacrificing valuable protein or peptide samples.
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Pierce™ Immunoprecipitation, Crosslink IP Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.
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Calmodulin Resin for CBP Purification, G-Biosciences
Supplier: G-Biosciences
The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.
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Recombinant Streptavidin, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli
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SDC™ (Steroid/Drug/Compound) Immobilization Kit, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification
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SNAP-CELL® Block , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells
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Pierce™ iodoTMTsixplex™ Isobaric Label Reagent Set, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific iodoTMTsixplex Label Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.
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Pierce™ DyLight™ Antibody Labeling, NHS Ester Quenchers, Thermo Scientific
Supplier: Thermo Scientific
DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes
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SNAP-SURFACE® Block , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells
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BG-Maleimide, New England Biolabs
Supplier: New England Biolabs (NEB)
BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides
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IMPACT™ Kit for Protein Purification, New England Biolabs
Supplier: New England Biolabs (NEB)
The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.
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Chitin Resin, New England Biolabs
Supplier: New England Biolabs (NEB)
An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.
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Streptavidin, New England Biolabs
Supplier: New England Biolabs (NEB)
A 52.8 kDa non-glycosylated protein with a very high affinity for biotin
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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific
Supplier: Thermo Scientific
Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.
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Pierce™ Immunostain Enhancer, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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Pierce™ Azide Labeled Sugars, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.