About this item
RNA from any sample (cells, tissues, biological fluids and more).
- DNA-free: Genomic DNA removal column and DNase I included
- High quality: Ensure intact RNA with DNA/RNA Shield™ for sample storage at ambient temperatures
- NGS-ready: RNA is ready for all downstream applications including next-gen sequencing, RT-qPCR, hybridization and more
The Quick-RNA™ miniprep plus kit is an innovative and versatile product designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield™ stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria and more). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17 to 200 nt).
Simply add DNA/RNA Shield™ and proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin™ column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing and more. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Total RNA
- Sample type:Cultured cellsHuman/animal cells and tissues
- Target molecule:RNA
- Sample size:≤1×107 Cells or 50 mg Tissue
- Description:Quick-RNA™ Miniprep Plus Kit
- Sample capacity:100 µg
- Yield:spin column, 100 µg RNA (binding capacity), ≥50 µl (elution volume)