AKT3 Western Bloting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Use Rockland Immunochemicals’ Anti-AKT3 Chemiluminescent Kit for Western Blotting for detection of human AKT3 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT3 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Type: Primary
Antigen: AKT3
Clonality: Monoclonal
Clone: 9A5.H9.G7
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity: