Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.

• 20X concentrated stock solution - DEPC Treated
• 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4
• Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers

The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Application Note: This product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4.  Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water treated with diethyl pyrocarbonate (DEPC).