Human Tenascin-C - TNC AccuSignal ELISA Kit

• Natural and recombinant human Tenascin-C.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: NSO; Immunogen sequence: G23-P625

Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 419pg/ml, 187.5pg/ml, 93.7pg/m human Tenascin-C standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human Tenascin-C standard solution and each sample be measured in duplicate.