Human TLR1 AccuSignal ELISA Kit

• Natural and recombinant human TLR1.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: NSO; Immunogen sequence: S22-N578

TLR1 is a member of the Toll-like receptor family(TLR) of pattern recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281(cluster of differentiation 281). TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human TLR1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TLR1 standard solution and each sample be measured in duplicate.