Human sVEGFR1 - sFLT1 AccuSignal ELISA Kit

• Natural and recombinant human sVegfr1.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: sf21; Immunogen sequence: S27-H687

sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human sVEGFR1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human sVEGFR1 standard solution and each sample be measured in duplicate.