DyLight™ Multiplex 649/549 Duo Western Blot Kit

• The DyLight™ multiplex Duo fluorescent Western blotting kit is suited for simultaneous detection and quantification of specific protein populations in a biological sample.
• The DyLight™ multiplex Duo Western blot kit contain all the necessary components that are optimized for the simultaneous detection of multiple proteins on the same blot using DyLight™-dye labeled secondary antibodies that are visualized in different fluorescence channels.
• The kit also includes blocking buffer, wash buffer, pre-stained protein standard and an incubation box for convinience and ultimate performance with minimal or no optimization.

Using a combination of two antibodies selected for minimal cross reactivity, fluorescent detection method enables simultaneous quantitative analysis of multiple proteins within the same sample on the same blot. The fluorescent dyes such as DyLights™ when conjugated to secondary antibodies, offer a variety of benefits over traditional detection methods such as colorimetric and chemiluminescent detection. Multiplex detection using the correct lighting and filter conditions, enables the quantitation of multiple proteins and eliminates the need to strip and reprobe. Other benefits of fluorescent Western blotting include increased sensitivity, excellent signal stability over time as well as precise quantitative analysis with broader dynamic range and high linearity. Due to their exceptional photostability, DyLight™ dye conjugates can be archived and visualized several times without a decrease in signal.

DyLight™ Multiplex 649/549 Duo Western Blot Kit contains Goat anti-mouse IgG (H+L) DyLight™ 549; Goat anti-rabbit IgG (H+L) DyLight™ 649; opal pre-stained protein standard; wash buffer (10x TTBS); blocking buffer (2x) and incubation box. This kit is suitable for fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial gel imaging systems.