RtcB Ligase
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- Return Policy
Product Details & Documents
RtcB Ligase is expressed as a His-tagged fusion in E. coli.
- This is an Enzyme for Innovation (EFI)
- These enzymes have interesting properties and unique specificities
RtcB Ligase from E. coli joins single stranded RNA with a 3'-phosphate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl. Ligation requires both GTP and MnCl₂ and proceeds through a 3'-guanylate intermediate. For substrates with a 2',3'-cyclic phosphate end, hydrolysis to a 3'-phosphate precedes 3' end activation with GMP and ligation.
Specifications
Specifications
- Concentration:15 µM
- Description:RtcB ligase
- No. of reactions:25
Specifications
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Catalog #: CA21905-026
Supplier: Kimberly-Clark