Simple, one-step solution for LAMP or RT-LAMP reactions.

• Fast, clear pink-to-yellow visible detection of amplification
• WarmStart feature inhibits activity at room temperature

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions.

This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15 to 40 minutes.

Application feature includes LAMP amplification of RNA and DNA targets, simple visual indication of amplification for easy detection and analysis, One-step field and point-of-need LAMP reactions and easy demonstration of LAMP assays with built-in detection.