Speed up with Chromolith® columns. Race through separations with revolutionary technology.

• Completely bioinert column hardware
• High biorecovery and high resistance to column blockage
• Selectivity for a range of biomolecules
• Very low column backpressure and high-speed separation possible
• Longer column lifetime
• Cost savings from higher sample throughput and column durability
• Possibility to use flow gradients

Affinity chromatography is a most selective technique which takes advantage of very specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. Analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products. Chromolith® WP 300 Protein A column could be used for separation of all IgGs (except class 3). Columns provides extremely fast separations and could be used longer minimizing.

To truly accelerate chromatographic separations, there's no better choice than our Chromolith® HPLC columns. Due to their revolutionary monolithic technology, Chromolith® columns provide excellent separations in a fraction of the time required by conventional particulate columns.

The secret to the speed of Chromolith® columns is their exceptionally low back pressure. Produced from a continuous piece of porous silica using a sol-gel process, Chromolith® columns possess a defined bimodal pore structure with macro and mesopores in the micro and nanometer range. The high permeability and porosity of the silica skeleton, and the resulting low back pressure allow for more flexible flow rates than particle-packed columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.

Each column consists of a single monolithic rod of high-purity polymeric silica gel with a revolutionary bimodal pore structure.