Certolizumab pegol ELISA detects precise determination of free Certolizumab pegol in serum & plasma.

• Dynamic Range: 6-200 ng/mL
• Sensitivity: 2 ng/mL
• Incubation: 3 hours

Certolizumab pegol (trade name Cimzia®) is a tumor necrosis factor alpha (TNFα) blocker and binds to human TNFα with a KD of 90pM. Certolizumab pegol is a recombinant, humanized antibody Fab' fragment, with specificity for TNFα, conjugated to an approximately 40kDa polyethylene glycol (PEG2MAL40K). The Fab' fragment is manufactured in E. coli and is subsequently subjected to purification and conjugation to PEG2MAL40K, to generate Certolizumab pegol. Steady-state concentrations range from 0.5 to 90 µg/mL for a fixed dose of 400 mg of certolizumab pegol.  The ImmunoGuide Certolizumab pegol ELISA  (mAb-based) kit can be efficiently used for measuring free Certolizumab pegol levels in serum and plasma.