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Specifications
- Size:500 Assays
- Cat. no.:103010-476
- Storage Temperature:– 20 °C
- Includes:The assays can be performed in a convenient 96-well microplate format, kit includes essential components and detailed protocol and references
- Supplier No.:AS-72136
Specifications
About this item
Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators.
The SensoLyte® Thiol Quantitation Assay Kit utilizes the widely used Elmann’s reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration.