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- Size:100 Assays
- Cat. no.:103010-618
- Storage Temperature:– 78 °C
- Includes:The assays can be performed in a convenient 96-well microplate format, kit includes essential components and detailed protocol and references
- Supplier No.:AS-72205
Nicotinamide nucleotides are key players in the energy transforming and oxidation-reduction reactions of a cell
The SensoLyte® NADP/NADPH Assay Kit utilizes an optimized enzymatic recycling method for the quantification of NADP/NADPH without interfering with NAD/NADH. The final product of reaction, formazan, has blue color and can be detected at absorbance 565 nm. The intensity of the color produced is proportional to NADP/NADPH concentration.
NADP and NADPH act as allosteric effectors in modulating enzyme activities. The reducing power of NADPH is a required cofactor for enzymes that are pro-oxidants such as nitric oxide synthase and NADPH oxidase. NADP/NADPH ratio is involved in the control of the oxidative shunt. Each of these forms has a particular role during differentiation and similarity processes as well as being target functions in the cytotoxic action of ionizing radiations and alkylating agents.