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- Size:100 Assays
- Cat. no.:103008-980
- Storage Temperature:– 78 °C
- Includes:The assays can be performed in a convenient 96-well microplate format, kit includes essential components and detailed protocol and references
- Supplier No.:AS-72243
ECEs or Endothelin converting enzymes (ECE-1/ECE-2) are known as rate-limiting enzymes for the formation of the vasoconstrictor peptide endothelin-1 (ET-1) from its precursor proendothelin-1
The SensoLyte® 520 ECEs Activity Assay Kit can be used to detect ECE activity in biological samples or in purified enzyme preparations. The substrate provided in this kit was based on a beta amyloid fragment. When active ECEs cleave the FRET substrate, it results in an increase of 5-FAM fluorescence, and is monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM shows less interference from autofluorescence of components in biological samples and test compounds. This assay can detect as low as 15.6 ng/mL active ECE-1 and 62.5 ng/mL active ECE-2.
ECE-1 can also degrade amyloid ß fragments both in vitro and in vivo. Highly homologous and structurally comparable to ECE-1, ECE-2 was shown in homozygous knockout mice to have decreased Aß degradation and significant elevated Aß 1-40 and Aß 1-42 in the brain.