Order Entry
Canada
ContactUsLinkComponent
SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit, AnaSpec Inc.
SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit, AnaSpec Inc.
Catalog # 103010-132
Supplier:  Anaspec Inc
CAS Number:  
SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit, AnaSpec Inc.
Catalog # 103010-132
Supplier:  Anaspec Inc
Supplier Number:  AS-71114
CAS Number:  

Some Products May Appear Restricted

To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.

If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/ca/en/login/register)

 

If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation;  additional documentation will be requested for these items prior to shipment. 

Specifications

  • Size:
    500 Assays (96-well format) or 1250 Assays (384-well format)
  • Cat. no.:
    103010-132
  • Storage Temperature:
    – 20 °C
  • Includes:
    The assays can be performed in a convenient 96-well or 384-well microplate format, kit includes essential components and detailed protocol and references
  • Supplier No.:
    AS-71114

Specifications

About this item

Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7

The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).