Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/ca/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Size:200 Assays
- Cat. no.:103008-978
- Storage Temperature:– 20 °C
- Includes:The assays can be performed in a convenient 96-well microplate format, kit includes essential components and detailed protocol and references
- Supplier No.:AS-72242
Specifications
About this item
The SensoLyte® 520 Acetylcholinesterase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of AChE inhibitors
Acetylcholinesterase (AChE) is a fast acting serine hydrolase that belongs to the carboxylesterase enzyme family. It hydrolyses acetylcholine into choline and acetic acid and is found in the synaptic cleft. It can be detected in many mammals and non-mammalian species. Alzheimer and myasthenia gravis diseases are characterized by reduced acetylcholine. Thus, drugs that inhibit AChE were developed to reduce disease symptoms. AChE can be inhibited by many natural substances such as alkaloids and synthetic chemicals such as organophosphorous compounds that are frequently used as pesticides. Recently, it was demonstrated that natural compounds have variable inhibitory effect on AChE depending on the species.
Active AChE generates fluorescence that can be monitored at excitation/emission=490/520 nm. The long wavelength fluorescence of the substrate in this kit minimizes interference from autofluorescence of components in biological samples and test compounds, resulting in higher sensitivity. Optimized to detect human AChE. Detects as low as 8 pg/mL of active AChE. Entire process can be completed in 1 hour.