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SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.
SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.
Catalog # 103010-664
Supplier:  Anaspec
CAS Number:  
SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.
Catalog # 103010-664
Supplier:  Anaspec
Supplier Number:  AS-72224
CAS Number:  

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Specifications

  • Size:
    96 Assays
  • Cat. no.:
    103010-664
  • Storage Temperature:
    –20 °C
  • Includes:
    Kit includes a 96-well plate, essential components and detailed protocol and references
  • Supplier No.:
    AS-72224

Specifications

About this item

The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. A monoclonal anti-human-MMP-2 antibody is employed to pull down MMP-2 from the mixture first. MMP-2 activity is then quantified by a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-2-induced cleavage of the FRET substrate. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. Ample materials are provided to perform 96 assays in a 96-well format.